Agakoresho ko gupima amaraso ka 0.25ml 0.5ml 1ml karimo agakoresho gato ko gupima amaraso karimo imiyoboro mito y'amaraso

Umuyoboro muto wo gukusanya amaraso ufite imiterere ya muntu n'umupfundikizo ufunze neza, ushobora gukumira amaraso kuva. Kubera ko ufite imiterere myinshi kandi ureba impande ebyiri, woroshye gutwara amaraso kandi ukayakoresha mu buryo bworoshye, nta maraso ashoka.

Ibara ry'umupfundikizo w'umutekano rijyanye n'amahame mpuzamahanga, byoroshye kumenya.

Igishushanyo mbonera cy’inkombe y’umuyoboro w’amaraso cyoroshye ku bakoresha mu gushyira amaraso mu muyoboro. Byoroshye, byihuse kandi bisobanutse, ingano y’amaraso ishobora gusomwa byoroshye hakoreshejwe umurongo usobanutse neza.

Uburyo bwihariye bwo kuvura imbere mu muyoboro, ni bworoshye hejuru nta maraso afatanye.

Ushobora guhindura barcode, no gukaraba umuyoboro w'amazi ukoresheje imirasire ya gamma nk'uko abakiriya babikeneye kugira ngo bapime asepsis.

1. Umuyoboro wa Gel & Clot Activator

Umuyoboro wa gel na clot activator bikoreshwa mu gusuzuma biochimie mu maraso, ubudahangarwa bw'umubiri no gupima imiti, n'ibindi. Aho hantu hasukwa coagulant ku buso buri imbere mu muyoboro, ibyo bikagabanya cyane igihe cyo kuvura.

Kubera ko jeli yo gutandukanya ivanwa mu Buyapani ari ikintu gisanzwe, ihamye cyane mu miterere ya fizikiki, ishobora kwihanganira ubushyuhe bwinshi ku buryo jeli izakomeza kuba nziza mu gihe cyo kuyibika no kuyitwara.

Iyo jeli izakomera nyuma yo kuyigabanya burundu, igatandukanya serum na fibrin nk'urukuta, ibyo bikarinda ihererekanya ry'ibintu hagati ya serum n'uturemangingo. Uburyo bwo gukusanya serum burushaho kuba bwiza kandi serum nziza izaboneka, bityo ikagera ku gipimo cy'ukuri.

Komeza serum idahinduka mu gihe kirenze amasaha 48, nta mpinduka igaragara izabaho ku miterere yayo ya biochemical n'ibinyabutabire, hanyuma umuyoboro ushobora gukoreshwa mu buryo butaziguye mu gupima ingero.

- Igihe cyo gukuraho burundu kw'amaraso mu mubiri: iminota 20-25
- Umuvuduko wo gukwirakwiza amashanyarazi: 3500-4000r/m
- Igihe cyo gukamura amashanyarazi: iminota 5
- Ubushyuhe busabwa mu kubika: 4-25ºC

2. Umuyoboro w'icyuma gitera amaraso

Umuyoboro w’amaraso ukoreshwa mu gukusanya amaraso mu bijyanye na biochimie na immunology mu isuzuma ry’abaganga. Ukwiriye ubushyuhe bwinshi bwo gukora. Iyo uvuwe by’umwihariko, ubuso bw’imbere bw’umuyoboro buba bworoshye cyane aho umuyoboro w’amaraso mwiza utera kimwe. Ingero z’amaraso zizahura burundu n’umuyoboro w’amaraso hanyuma zigaterana mu minota 5-8. Bityo, serum nziza iboneka nyuma yo gukwirakwiza amaraso mu buryo bwa centrifuge, nta gucika kw’amaraso, hemolysis, gutandukanya poroteyine ya fibrin, nibindi.

Bityo seramu ishobora kuzuza ibisabwa byo gupima seramu byihuse no gupima mu buryo bwihutirwa.
- Igihe cyo gukuraho burundu kw'amaraso mu mubiri: iminota 20-25
- Umuvuduko wo gukwirakwiza amashanyarazi: 3500-4000r/m
- Igihe cyo gukamura amashanyarazi: iminota 5
- Ubushyuhe busabwa mu kubika: 4-25ºC

3. Umuyoboro wa EDTA

Umuyoboro wa EDTA ukoreshwa cyane mu buvuzi bw'amaraso, guhuza amaraso, gushyira amaraso mu byiciro bitandukanye ndetse no mu bwoko butandukanye bw'ibikoresho byo gupima uturemangingo tw'amaraso.

Itanga uburinzi bwuzuye ku turemangingo tw’amaraso, cyane cyane mu kurinda uduce duto tw’amaraso, kugira ngo ibashe guhagarika guterana kwa platelets z’amaraso no gutuma imiterere n’ingano y’uturemangingo tw’amaraso bitagerwaho mu gihe kirekire.

Imyenda myiza cyane ifite ubuhanga bwa super-minute ishobora gutera inyongeramusaruro ku buso bw'imbere bw'umuyoboro, bityo amaraso ashobora kuvanga neza n'inyongeramusaruro. Plasma ya EDTA irwanya amaraso ikoreshwa mu gusuzuma mikorobe zitera indwara, udukoko n'udukoko twa bagiteri, nibindi.

4. Umuyoboro wa ADN

1. Umuyoboro wa RNA/DNA y'amaraso wuzuyemo reagent yihariye kugira ngo urinde vuba RNA/DNA y'ibipimo kugira ngo idakwirakwira

2. Ingero z'amaraso zishobora kubikwa iminsi 3 kuri 18-25°C, zikabikwa iminsi 5 kuri 2-8°C, zikagumana ubushyuhe byibuze amezi 50 kuri -20°C kugeza -70°C.

3. Byoroshye gukoresha, hindura gusa umuyoboro wa RNA/DNA y'amaraso inshuro 8 nyuma yo gufata amaraso menshi.

4. Shyira ku maraso mashya y'abantu n'inyamabere, ntabwo bikwiriye amaraso yangiritse n'amaraso ahumye ndetse n'amaraso y'inkoko n'andi matungo.

5. Gukusanya, kubika no gutwara ingero z’amaraso yose zipima RNA/DNA

6. Urukuta rw'imbere rw'umuyoboro ni umwihariko wo gutunganya nta RNase, DNase, rutuma ingero zo gupima aside nucleic zigaragara neza.

7. Bifasha mu gukura ingero nyinshi no kuzikura vuba, binoza imikorere ya laboratwari

5. Umuyoboro wa ESR

Umuyoboro wa ESR wa Ø13×75mm ukoreshwa cyane mu gukusanya no kurwanya amaraso mu gupima igipimo cy’amaraso ya Erythrocyte Sedimentation Rate Analyzers hakoreshejwe uburyo bwa Westergren bwo kuvanga igice kimwe cya sodium citrate n’ibice bine by’amaraso.

6. Umuyoboro wa glucose

Umuyoboro wa glucose ukoreshwa mu gukusanya amaraso mu gupima nk'isukari mu maraso, kwihanganira isukari, electrophoresis ya erythrocyte, hemoglobini irwanya alkali na lactate. Sodium Fluoride yongewemo irinda neza imikorere y'isukari mu maraso, naho Sodium Heparin igakemura neza ikibazo cyo kuva amaraso mu maraso.

Bityo, uko amaraso yari ameze mbere bizamara igihe kirekire kandi bitanga amakuru ahamye ku isukari yo mu maraso mu masaha 72. Inyongera y'ibikenewe ni Sodium Fluoride + Sodium Heparin, Sodium Fluoride + EDTA.K2, Sodium Fluoride + EDTA.Na2.

Umuvuduko wo gupima centrifugation: 3500-4000 r/m
Igihe cyo gupima centrifugation: iminota 5
Ubushyuhe busabwa mu kubika: 4-25 ºC

7. Umuyoboro wa Heparin
Umuyoboro wa Heparin ukoreshwa mu gukusanya amaraso mu gupima plasma yo kwa muganga, biochimie yihutirwa na rheology y'amaraso, nibindi. Kubera ko nta kintu kinini kibangamiye imiterere y'amaraso kandi nta ngaruka igira ku bunini bwa erythrocyte, ntabwo itera hemolysis. Byongeye kandi, ifite imiterere yo gutandukana vuba kwa plasma n'ubushyuhe bwinshi bw'imikorere ndetse no guhuza neza n'igipimo cy'amaraso mu maraso.

Heparin irwanya amaraso agabanuka ikora fibrinolysin, mu gihe igabanya thromboplastin, hanyuma ikagera ku buringanire hagati ya fibrinogen na fibrin, nta mugozi wa fibrin uriho mu isuzuma. Ibyinshi mu bipimo bya plasma bishobora gusubirwamo mu masaha 6.

Heparin ya Lithium ntabwo ifite gusa imiterere ya heparin ya sodium, ahubwo irashobora no gukoreshwa mu isuzuma ry'ibintu bito nta ngaruka ku iyoni ya sodium. Kugira ngo habeho ibyo laboratwari y'ubuvuzi ikeneye, KANGJIAN ishobora kongeramo gel yo gutandukanya plasma kugira ngo ikore plasma nziza.

Umuvuduko wo gupima centrifugation: 3500-4000 r/m
Igihe cyo gupima centrifugation: iminota 3
Ubushyuhe busabwa mu kubika: 4-25ºC

Umuyoboro wa PT 8

Umuyoboro wa PT ukoreshwa mu gupima amaraso agwa kandi ukoreshwa mu buryo bwa fibrinolytic (PT, TT, APTT na fibrinogen, nibindi).
Igipimo cyo kuvanga ni igice kimwe cya citrate ku bice 9 by'amaraso. Igipimo nyacyo gishobora kwemeza ko ibisubizo by'ikizamini bizagira ingaruka nziza no kwirinda ko isuzuma ritari ryo.

Kubera ko sodium citrate ifite uburozi buke cyane, ikoreshwa no mu kubika amaraso. Kuramo amaraso ahagije kugira ngo urebe neza ibisubizo by'ikizamini. Umuyoboro wa PT ufite ahantu habiri ufite umwanya muto, ushobora gukoreshwa mu gukurikirana ikizamini cya v WF, F, imikorere ya platelet, na Heparin.