Imiyoboro yo gukusanya amaraso ya EDTA ni iki kandi ikoreshwa ite?

Mu gupima no gusuzuma no kuvura indwara,imiyoboro yo gukusanya amaraso ya EDTA, nk'ibikoresho by'ingenzi byo gukusanya amaraso, bigira uruhare runini mu kwemeza ubuziranenge bw'ibipimo n'ukuri kw'ibipimo. Muri iyi nkuru, tuzasesengura byimbitse iyi "murinzi utagaragara" mu rwego rw'ubuvuzi duhereye ku bisobanuro, ishyirwa mu byiciro ry'amabara, ihame ryo kurwanya amaraso agabanuka, intego yo gupima n'uburyo bwo gukoresha.

Ni ikiUmuyoboro wo gukusanya amaraso wa EDTA?

Umuyoboro wo gukusanya amaraso wa EDTA ni ubwoko bw'umuyoboro wo gukusanya amaraso urimo aside Ethylene Diamine Tetraacetic cyangwa umunyu wayo, ukoreshwa cyane cyane mu gukusanya ingero z'amaraso no kuvura indwara yo kugabanya amaraso. EDTA ishobora guhagarika uburyo amaraso agwa binyuze mu gukata iyoni za kalisiyumu mu maraso, kugira ngo amaraso agume mu buryo bw'amazi igihe kirekire, kandi igatanga ingero zihamye zo gupima amaraso asanzwe n'ibinyabuzima by'umubiri. Itanga ingero zihamye zo gupima amaraso asanzwe, ibinyabuzima by'umubiri n'ibindi bipimo.

Nk'igice cy'ingenzi cyaibikoresho byo kwa muganga, imiyoboro yo gukusanya amaraso ya EDTA igomba kubahiriza amahame ngenderwaho y’igihugu agenga “Ibikoresho byo gukusanya amaraso mu mitsi bikoreshwa rimwe” (urugero: GB/T 19489-2008) kugira ngo habeho imikorere myiza y’ubutabire, ubutavangira kandi butari uburozi.

Amabara atandukanye y'imiyoboro yo gukusanya amaraso ya EDTA

Dukurikije amahame mpuzamahanga asanzwe (nk'amabwiriza ya CLSI H3-A6), imiyoboro yo gukusanya amaraso ya EDTA ikunze kuba ipfundikiye mu ibara ry'umuhengeri (EDTA-K2/K3) cyangwa ubururu (sodium citrate ivanze na EDTA) kugira ngo hamenyekane ikoreshwa:

Icyitonderwa: Hari ubwoko bumwe na bumwe bushobora kuba bufite andi mabara, banza urebe amabwiriza mbere yo gukoresha.

Uburyo bwo kurwanya amaraso ya EDTA mu miyoboro yo gukusanya amaraso

EDTA binyuze mu itsinda ryayo rya molekile karubokisili (-COOH) na iyoni za kalisiyumu mu maraso (Ca²⁺) bihujwe bigakora chelate ihamye, bityo bikabuza imikorere ya plasminogen, bikabuza inzira yo gufungana kwa fibrinogen muri fibrin. Iyi anticoagulation ifite ibi bikurikira:

1. gutangira vuba gukora: imiti igabanya amaraso ishobora kurangira mu minota 1-2 nyuma yo gufata amaraso;

2. ubusugire bukabije: ingero zishobora kubikwa amasaha arenga 48 (muri firigo zishobora kongerwa kugeza ku masaha 72);

3. Uburyo bwinshi bwo gukoresha: bukwiriye ibizamini byinshi byo mu maraso, ariko ntabwo bukwiriye ibizamini byo gufungana cyangwa imikorere ya platelet (imiyoboro ya sodium citrate irakenewe).

Ibintu by'ingenzi byo gupima mu muyoboro wo gukusanya amaraso wa EDTA

1. isesengura ry’amaraso risanzwe: umubare w’uturemangingo tw’amaraso twera, ibipimo by’uturemangingo dutukura tw’amaraso, urugero rwa hemoglobin, nibindi;

2. kumenya no guhuza amatsinda y'amaraso: Itsinda ry'amaraso rya ABO, Gupima Rh factor;

3. gusuzuma molekile: gupima imiterere y'uturemangingo, kumenya umubare w'udukoko twa virusi (urugero: VIH, HBV);

4. hemoglobine ya glycated (HbA1c): kugenzura isukari mu maraso igihe kirekire kuri diyabete;

5. Gupima udukoko tw’amaraso: Plasmodium, gusuzuma microfilariae.

Gukoresha amahame n'uburyo bwo kwirinda

1. Uburyo bwo gukusanya:

Nyuma yo kwisiga imiti yica uruhu, kora ukurikije uburyo bwo gufata amaraso mu mitsi;

Ukimara gufata amaraso, hindura umuyoboro wafatiwemo amaraso inshuro 5-8 kugira ngo urebe neza ko umuti ugabanya amaraso uvanze neza n'amaraso;

Irinde kunyeganyega cyane (kugira ngo wirinde ko amaraso asohoka).

2. Kubika no gutwara:

Bika ahantu hari ubushyuhe bw'icyumba (15-25°C), wirinde ubushyuhe cyangwa gukonjesha;

Shyira uhagaze mu gihe cyo gutwara kugira ngo wirinde ko umupfundikizo w'umuyoboro urekura.

3. Ingero z'ibibujijwe:

Imiyoboro ya sodium citrate irakenewe kugira ngo umubiri ugire ubwivumbure bwa IV (PT, APTT, nibindi);

Ikizamini cy'imikorere y'imitsi gisaba umuyoboro wa sodium citrate.

Uburyo bwo guhitamo ubuziranengeUmuyoboro wo gukusanya amaraso wa EDTA?

1. Impamyabushobozi n'impamyabushobozi: hitamo ibicuruzwa byatsinze icyemezo cya ISO13485 na CE. 2;

2. Umutekano w'ibikoresho: igice cy'umuyoboro kigomba kuba gifite urumuri kandi kidafite ibisigazwa bya pulasitiki;

3. Gupima neza: ingano y'umuti ugabanya amaraso wongewemo igomba kuba ijyanye n'ibipimo ngenderwaho by'igihugu (urugero: igipimo cya EDTA-K2 cya 1.8±0.15mg/mL);

4. Izina ry'ikirango: Ibigo bizwi cyane mu bijyanye n'ibikoresho by'ubuvuzi bihabwa umwanya wa mbere kugira ngo habeho ihinduka ry'ibicuruzwa.

Umwanzuro

Nk'umunyamuryango w'ingenzi waigikoresho cyo gukusanya amaraso, imiyoboro yo gukusanya amaraso ya EDTA igira ingaruka zitaziguye ku buryo ibisubizo by’ibizamini bifatika bingana n’imiterere yayo irwanya amaraso ku mubiri. Mu gushyiraho uburyo busanzwe bwo gukoresha imiyoboro itandukanye yo gukusanya amaraso ifite amabara atandukanye no kuyahuza n’uburyo buhamye bwo kuyakusanya, bishobora gutanga ishingiro ryizewe ryo gusuzuma indwara. Mu gihe kizaza, hamwe n’iterambere ry’ubuvuzi bunoze, imiyoboro yo gukusanya amaraso ya EDTA izagira uruhare runini mu gusesengura amaraso, gukurikirana ingirabuzima fatizo n’ibindi bice, kandi ikomeze kurinda ubuzima bw’abantu.