Niki Amaraso yo gukusanya Amaraso ya EDTA kandi akoreshwa gute?

Mu kwipimisha kwa muganga no gusuzuma no kuvura,EDTA yo gukusanya amaraso, nk'ibikoresho by'ingenzi bikoreshwa mu gukusanya amaraso, bigira uruhare runini mu kwemeza ubusugire bw'icyitegererezo no kwipimisha neza. Muri iki kiganiro, tuzasesengura byimazeyo uyu "murinzi utagaragara" murwego rwubuvuzi duhereye kubisobanuro, gutondekanya amabara, ihame rya anticoagulation, intego yo gupima no gukoresha ibipimo.

NikiEDTA yo gukusanya amaraso?

Umuyoboro wo gukusanya amaraso wa EDTA ni ubwoko bwikusanyirizo ryamaraso ya vacuum irimo Acide ya Ethylene Diamine Tetraacetic Acide cyangwa umunyu wacyo, ikoreshwa cyane mugukusanya amaraso no kuvura anticoagulant. EDTA irashobora guhagarika coagulation cascade reaction mugukata calcium ya calcium mumaraso, kugirango amaraso agume mumazi igihe kirekire, kandi atange ingero zihamye zo gupima gahunda zamaraso na biologiya ya molekuline. Itanga ingero zifatika kumikorere yamaraso, ibinyabuzima bya molekuline nibindi bizamini.

Nkigice cyingenzi cyaibikoresho byo kwa mugangaImiyoboro yo gukusanya amaraso ya EDTA igomba kubahiriza igipimo cyigihugu cy '“Gukoresha inshuro imwe gusa yo gukusanya amaraso yo mu maraso” (urugero: GB / T 19489-2008) kugirango harebwe imikorere yubusembwa, butari pyrogenic na non-cytotoxicity.

Amabara atandukanye ya EDTA yo gukusanya amaraso

Ukurikije amahame mpuzamahanga asanzwe (nk'amabwiriza ya CLSI H3-A6), imiyoboro yo gukusanya amaraso ya EDTA ikunze gufatwa mubururu (EDTA-K2 / K3) cyangwa ubururu (citrate sodium ivanze na EDTA) kugirango itandukanye ikoreshwa:

Icyitonderwa: Ibirango bimwe bishobora kwandikwa mumabara yandi, reba amabwiriza mbere yo gukoresha.

Uburyo bwo kurwanya anticagulation ya EDTA yo gukusanya amaraso

EDTA ibinyujije mu itsinda ryayo rya karubike (-COOH) hamwe na calcium ion mu maraso (Ca²⁺) byahujwe no gukora chelate ihamye, bityo bikabuza gukora plasminogene, bikabuza uburyo bwo guhuza fibrinogene muri fibrine. Iyi anticoagulation ifite ibintu bikurikira:

1. Gutangira ibikorwa byihuse: anticoagulation irashobora kurangira muminota 1-2 nyuma yo gukusanya amaraso;

2. Umutekano mwinshi: ingero zirashobora kubikwa mumasaha arenga 48 (firigo irashobora kongerwa kumasaha 72);

3. Urwego runini rushyirwa mu bikorwa: rukwiriye kwipimisha indwara nyinshi, ariko ntabwo ari ibizamini bya coagulation cyangwa platelet (sodium citrate tubes irakenewe).

Ibikoresho byingenzi byo gupima amaraso ya EDTA

1.

2. Kumenyekanisha amatsinda yamaraso no guhuza: ABO itsinda ryamaraso, Rh factor detection;

3. Gusuzuma molekuline: gupima genetike, kugena imitwaro ya virusi (urugero: VIH, HBV);

4.

5. Kwipimisha amaraso parasite: Plasmodium, kumenya microfilariae.

Gukoresha amahame no kwirinda

1. Uburyo bwo gukusanya:

Nyuma yo kwanduza uruhu, kora ukurikije igipimo cyo gukusanya amaraso;

Ako kanya nyuma yo gukusanya, hindura umuyoboro wamaraso inshuro 5-8 kugirango umenye ko anticoagulant ivanze namaraso;

Irinde kunyeganyega bikabije (kugirango wirinde hemolysis).

2. Kubika no gutwara:

Bika ku cyumba cy'ubushyuhe (15-25 ° C), irinde ubushyuhe cyangwa ubukonje;

Shyira uhagaritse mugihe cyo gutwara kugirango wirinde kurekura umupira.

3. Ibintu byo kurwanya ibicuruzwa:

Sodium citrate tubes irakenewe kuri Coagulation IV (PT, APTT, nibindi);

Ikizamini cyimikorere ya platelet gisaba sodium citrate tube.

Nigute wahitamo ubuziranengeEDTA yo gukusanya amaraso?

1. Impamyabumenyi n'impamyabumenyi: hitamo ibicuruzwa byatsinze ISO13485 na CE. 2;

2. Umutekano wibikoresho: umubiri wigituba ugomba kuba mucyo kandi udafite ibisigazwa bya plastike;

3. Kunywa neza: ingano ya anticoagulant yongeweho igomba kuba ijyanye nuburinganire bwigihugu (urugero EDTA-K2 yibanda kuri 1.8 ± 0.15mg / mL);

4.

Umwanzuro

Nkumunyamuryango wingenzi waigikoresho cyo gukusanya amaraso, EDTA yo gukusanya amaraso igira ingaruka itaziguye kubisubizo byibizamini ukurikije imiterere yabyo. Muguhuza ikoreshwa ryimiyoboro itandukanye yo gukusanya amaraso no kuyahuza nuburyo bukomeye bwo gukusanya, birashobora gutanga ishingiro ryizewe ryo gusuzuma indwara. Mu bihe biri imbere, hamwe n’iterambere ry’ubuvuzi bwuzuye, imiyoboro yo gukusanya amaraso ya EDTA izagira uruhare runini mu gusesengura amaraso, uko gene ikurikirana ndetse n’izindi nzego, kandi ikomeze kurengera ubuzima bw’abantu.